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Subfamily Tribe Genus Specific epithet Infraspecific taxa
2C value -   Ploidy -   Chrom. number -   Method  
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Cytogenetic characterization and nuclear DNA content of three North African endemic Centaurea species

Benamara-Bellagha, M., Baziz, K., Pustahija, F., Khalfallah, N., & Siljak-Yakovlev, S. (2014). Cytogenetic characterization and nuclear DNA content of three North African endemic Centaurea species. Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology, (just-accepted), 1-23.

TCentaurea_pullata_bhree endemic Centaurea species from North Africa are investigated for the first time by chromomycin fluorochrome banding for GC-rich DNA distribution, fluorescence in situ hybridization (FISH) for physical mapping of rRNA genes, and flow cytometry for genome size assessment. Investigated species belong to three different sections and possess three basic chromosome numbers: C. tougourensis subsp. tougourensis2n = 4x = 36 (x = 9), C. musimonum 2n = 2x = 20 (x = 10) and C. maroccana 2n = 2x = 24 (x = 12). The number and distribution of chromomycin positive bands (CMA+) and 18S-5.8S-26S (35S) rDNA loci were different among investigated species and ranged from 6 to 80 chromomycin bands and from 2 to 6 35S rDNA loci. The three species have just one 5S rDNA locus at intercalary position on a separate chromosome pairs, except in the case of C. musimonum in which both rDNA loci were localized on the same chromosome. All rDNA loci were co-localized with CMA+ bands, except three 35S in C. musimonum. Genome size ranged from 2C = 1.66 to 2C = 2.86 pg in diploid species (C. musimonum and C. maroccanarespectively) and to 2C = 4.51 pg in tetraploid C. tougourensis subsp. tougourensis.

Paper can be accessed here

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